Molecular dynamics simulation is applied to study the instability and rupture process of ultra-thin water films on a solid substrate. Results show the small disturbance of the film will develop linearly due to the spinodal instability, whereas the interaction between solid and liquid has less influences on the initial growth. Then the rupture occurs and the rim recedes with a dynamic contact angle. The radius of the rim varies with time as the square root of t, which is consistent with the macroscopic theory available. Stronger interaction between solid and liquid will postpone rupture time, decline the dynamic contact angle and raise the density of water near the interface between solid and liquid.