According to the vibration characteristics of the organ of Corti (OC), seven hypotheses are made to simplify the structure of the model, and a mechanical OC model is established. Using the variational principle, a displacement analytical expression is solved under a certain pressure. The results are in good agreement with experimental data, showing the validity of the formula. Combined with the damage caused by noise in clinic, it is found that the hardening of outer hair cells and outer stereocilia can lead to loss of hearing and generation of threshold shift. In addition, the results show that high frequency resonance occurs at the bottom of the basilar membrane (BM), and low frequency resonance occurs at the top of the BM. This confirms the frequency selective characteristics of the BM. Further, using this formula can avoid interference of the envi-ronment and the technical level of the test personnel, and can evaluate performance of the OC objectively.